| 产品中文名称 | 重组小鼠白介素-4（无动物源）（IL-4) |
| 产品英文名称 | Mouse IL-4 protein |
| 产品别名 | B-cell lgG differentiation factor; B-cell growth factor 1; B-cell stimulatory factor 1 (BSF-1); lGG1 induction factor; Lymphocyte stimulatory factor 1 |
| 产品描述 | Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and for the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as of other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibites in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and of the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells. |
| 种属 | 小鼠 |
| 表达宿主 | 大肠杆菌 |
| 活性 | 在使用NFS-60的细胞增殖测定中测量。该效应的ED 50通常为5.37 ng/mL。重组小鼠IL 4的比活性约为>1.86 x 105 IU/mg。 |
| 蛋白长度 | 13.4 kDa |
| 纯度 | > 95% |
| 产品形式 | 冻干粉，冻干缓冲液为无菌的PBS , pH 7.4。 |
| 特点 | 通过LAL法测定，每微克蛋白里内毒素含量<1 EU。 |
| 使用中注意事项 | 开盖使用前，请先离心。本产品为冻干粉，推荐用该产品配套的Reconstitution Buffer进行复溶，且浓度不低于100 µg/mL。客户可根据后续的实验需求，进行进一步的稀释。 |
| 保存建议 | 冻干蛋白应在 -20°C 下保存 1 年。复溶后，在 2°C 至 8°C 下最多可保存 1 周。 在含有载体蛋白或稳定剂的缓冲液（如 0.1% BSA、10%FBS、5%HSA）中进一步稀释后，分装的蛋白质应在 -20°C 或 -80°C 下保存 3-6 个月。避免反复冻融循环。 |
| 运输条件 | 冰袋运输（蓝冰） |
| 警告 | 本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。 |
| 基因ID | 16189 |