| 中文产品名称 | 重组小鼠粒细胞集落刺激因子（G-CSF) |
| 英文产品名称 | Mouse G-CSF protein |
| 产品别名 | G-CSF; Colony Stimulating Factor 3; Granulocyte; GCSF |
| 产品描述 | Granulocyte-colony stimulating factor (G-CSF) is a growth factor and an essential cytokine belonging to the CSF family of hormone-like glycoproteins. It is produced by numerous cell types including immune and endothelial cells. G-CSF binding to its receptor G-CSF-R which belongs to the cytokine receptor type I family depends on the interaction of alpha-helical motifs of the former and two fibronectin type III as well as an immunoglobulin-like domain of the latter. Recent animal studies have also revealed that G-CSF activates multiple signaling pathways, such as Akt and also the Janus family kinase-2 and signal transducer and activation of transcription-3 (Jak2-STAT3) pathway, thereby promoting survival, proliferation, differentiation and mobilisation of haematopoietic stem and progenitor cells. G-CSF is a cytokine that have been demonstrated to improve cardiac function and perfusion in myocardial infarction. And it was initially evaluated as a stem cell mobilizer and erythropoietin as a cytoprotective agent. G-CSF prevents left ventricular remodeling after myocardial infarction by decreasing cardiomyocyte death and by increasing the number of blood vessels, suggesting the importance of direct actions of G-CSF on the myocardium rather than through mobilization and differentiation of stem cells. Accordingly, recombinant human (rh)G-CSF has been extensively used in clinical haematology and oncology to enable bone marrow transplantation or to treat chemotherapy-associated neutropenia. In preclinical study, G-CSF improved cardiac function and perfusion by angiomyogenesis and protection of cardiomyocytes in myocardial infarction. |
| 种属 | 小鼠 |
| 表达宿主 | 大肠杆菌 |
| 分子 | G-CSF; Colony Stimulating Factor 3; Granulocyte; GCSF |
| 检测指标 | G-CSF; Colony Stimulating Factor 3; Granulocyte; GCSF |
| 活性 | Measured by its ability to induce proliferation in M-NFS-60 cells. The ED50 for this effect is 0.03 ng/mL. The specific activity of recombinant Mouse G-SCF is approximately >3.33 x 10 7 IU/mg. |
| 蛋白长度 | 重组小鼠粒细胞集落刺激因子（G-CSF) 由178个氨基酸组成，预测分子量为18.95 KD。 |
| 纯度 | > 99% ，使用SDS-PAGE检测 |
| 产品形式 | 冻干粉，冻干缓冲液为无菌的20 mM Tris，150 mM NaCl，pH 7.4。 |
| 特点 | 通过LAL法测定，每微克蛋白里内毒素含量<1.0 EU。 |
| 分子量 | 18.95 KD |
| 使用中注意事项 | 开盖使用前，请先离心。本产品为冻干粉，推荐用该产品配套的Reconstitution Buffer进行复溶，且浓度不低于100 µg/mL。客户可根据后续的实验需求，进行进一步的稀释。 |
| 保存建议 | 本产品为冻干粉形式，建议：将冻干粉产品保存于-20℃以下的干燥环境中。本产品溶解之后，可在4℃保存2-7天，长期使用请置于-20℃以下。为长期保存，推荐添加载体蛋白（0.1% HAS或BSA），并酌情分装保存，避免反复冻融。 |
| 运输条件 | 冰袋运输（蓝冰） |
| 警告 | 本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。 |
| 基因ID | 12985 |
| 蛋白质ID | P09920 |